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However it should be appreciated that these sensors are recording the lactate outdoors the nerve, so the interstitial focus is more likely to be considerable higher. In addition, given the lack of lactate in the aCSF, there's a really massive focus gradient forcing lactate out of the tissue, which differs from the in vivo situation where there may be a constant lactate presence within the interstitium. A gentle recording of lactate implies a relentless efflux from the tissue as we are in effect recording a web flux, relatively than a static concentration, for the reason that nerve is frequently superfused with aCSF at a charge of 2 ml min-1. These data do indeed suggest that astrocytes launch lactate that is accessible to the axons for uptake (Yang et al., 2014), nevertheless it must be appreciated that below such circumstances there's a large gradient for lactate to move from the interstitium out of the nerve as there is no lactate in the aCSF superfusing the nerve.
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